FastQCFastQC Report
Thu 2 Jun 2016
DRR015392_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015392_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11763420
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448370.3811561603683282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371920.3161665570046806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203690.17315542588804955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA234750.054.979181
GTATCAA433700.040.986461
TCAACGC532550.033.025954
ATCAACG533600.032.99623
CAACGCA550750.031.8106445
TATCAAC560200.031.4062732
AACGCAG569100.030.8684396
ACGCAGA636150.027.2358477
CGCAGAG633100.027.0922328
GTGGTAT94600.025.2079641
GTACATG348750.025.015291
GCAGAGT668500.024.744149
TACATGG339200.023.6668432
TGGTATC100150.022.9930822
ACATGGG354100.022.0660923
GAGTACT453200.021.63777712-13
CAGAGTA647600.021.24722510-11
AGAGTAC604000.020.60417710-11
GTACTTT482950.020.10230414-15
AGTACTT462050.019.44610812-13
Produced by FastQC (version 0.11.5)