FastQCFastQC Report
Thu 2 Jun 2016
DRR015392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11763420
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449690.38227828301633365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341560.2903577361005558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238860.20305319371407296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221050.053.5047261
GTATCAA434250.042.4847531
ATCAACG549650.033.3200233
TCAACGC549900.033.2467964
CAACGCA566200.032.2483335
TATCAAC576600.031.8185922
AACGCAG587250.031.1534446
ACGCAGA663800.027.1843997
CGCAGAG660550.027.101658
GTACATG336450.024.973911
GCAGAGT699750.024.828739
TACATGG329600.024.2068062
GTGGTAT97450.023.9275821
TGGTATC99000.022.572992
ACATGGG345200.022.1894683
AGAGTAC637050.021.44442610-11
GAGTACT481200.021.08478712-13
TATAACG11450.021.0675092
AGTACTT499000.020.30530712-13
CAGAGTA683400.020.2097510-11
Produced by FastQC (version 0.11.5)