FastQCFastQC Report
Thu 2 Jun 2016
DRR015391_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015391_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10193149
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402950.395314539206677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332970.32666058349583627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180440.17702085979514282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194000.057.4949871
GTATCAA362950.041.802971
ATCAACG446600.033.574053
TCAACGC448550.033.4784
TATCAAC464150.032.3453872
CAACGCA464300.032.3227585
AACGCAG479100.031.4057396
GTGGTAT72400.028.5368271
ACGCAGA535650.027.6229747
CGCAGAG540200.027.160778
TGGTATC76500.025.9366232
GCAGAGT575250.024.6979549
GTACATG311550.023.7217461
TATAACG9900.023.443552
TACATGG310300.022.4240552
GAGTACT388900.021.6705612-13
CAGAGTA556150.021.32548910-11
ACATGGG321700.020.935233
AGAGTAC525150.020.62218710-11
GTACTTT417800.019.88845814-15
Produced by FastQC (version 0.11.5)