FastQCFastQC Report
Thu 2 Jun 2016
DRR015391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10193149
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392220.3847878609446404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306060.3002604984975693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209940.20596186713252207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181000.057.041481
GTATCAA357450.044.567921
ATCAACG461850.034.056893
TCAACGC463450.033.938314
CAACGCA476250.033.1033945
TATCAAC478100.032.9759752
AACGCAG492700.032.1264956
ACGCAGA557200.027.9435587
CGCAGAG558950.027.6311248
GTGGTAT71700.026.1060121
GCAGAGT593350.025.2774839
TGGTATC74900.023.6985242
GTACATG291200.022.458511
AGAGTAC547550.021.81166610-11
GAGTACT408750.021.2586912-13
CAGAGTA580200.020.76463710-11
TACATGG292600.020.703432
AGTACTT425800.020.43416212-13
GTACTTT442350.019.304214-15
ACATGGG306300.018.841023
Produced by FastQC (version 0.11.5)