FastQCFastQC Report
Thu 2 Jun 2016
DRR015390_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015390_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12423937
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413270.3326401284874513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286530.2306273768130022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154880.12466257676612494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200450.055.2135851
GTATCAA421350.045.6970831
ATCAACG499550.038.0779463
TCAACGC499500.038.04444
TATCAAC522650.036.552742
CAACGCA520050.036.5410585
AACGCAG540200.035.2831466
ACGCAGA595300.031.3600837
CGCAGAG598150.030.859138
GTGGTAT77950.028.6651251
GCAGAGT633450.028.1822559
TGGTATC85700.025.1170582
GTACATG351750.023.585281
TACATGG349200.022.4412672
GAGTACT426200.021.88276112-13
CAGAGTA616600.021.75883510-11
AGAGTAC581800.021.23442310-11
ACATGGG356000.021.0660633
GTACTTT457500.020.05239314-15
TATAACG10300.019.882172
Produced by FastQC (version 0.11.5)