FastQCFastQC Report
Thu 2 Jun 2016
DRR015390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12423937
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405070.32603996623614556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258580.2081304823100761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174650.14057540697445584No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186650.055.2617951
GTATCAA404650.046.231261
ATCAACG502250.036.896253
TCAACGC502950.036.8354074
CAACGCA520550.035.6505935
TATCAAC524850.035.386682
AACGCAG541450.034.324626
ACGCAGA610200.029.9367497
CGCAGAG614900.029.4459388
GCAGAGT651150.026.6603579
GTGGTAT77050.025.6508581
GTACATG339050.023.0214081
TGGTATC83300.022.8370342
AGAGTAC600350.021.56120910-11
TACATGG338150.021.5606332
GAGTACT444200.020.64336212-13
CAGAGTA637350.020.2988110-11
ACATGGG343750.020.2819883
AGTACTT463200.019.83097812-13
GTACTTT479400.018.89991214-15
Produced by FastQC (version 0.11.5)