FastQCFastQC Report
Thu 2 Jun 2016
DRR015389_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015389_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16653189
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT553790.33254291415295895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409020.2456106154803143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226690.13612407809699392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA272500.055.7772251
GTATCAA541700.044.6331941
ATCAACG661000.036.1082653
TCAACGC661350.036.0472224
TATCAAC686100.034.8415072
CAACGCA693450.034.3523375
AACGCAG722750.033.056286
ACGCAGA792250.029.6108367
CGCAGAG794050.029.2789048
GTGGTAT104750.027.673391
GCAGAGT855800.026.3888479
GTACATG479050.024.8789251
TACATGG477450.023.3896452
TGGTATC114600.023.3490732
ACATGGG497500.021.604763
GAGTACT578500.021.44601412-13
CAGAGTA838500.021.17944710-11
AGAGTAC788200.020.90333610-11
GTACTTT629450.019.540314-15
AGTACTT599250.019.48109812-13
Produced by FastQC (version 0.11.5)