FastQCFastQC Report
Thu 2 Jun 2016
DRR015389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16653189
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT539790.324136115911493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376850.22629299409260292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264230.15866630709589616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA264500.054.509461
GTATCAA542200.045.9340821
ATCAACG688550.035.80863
TCAACGC692100.035.5855874
CAACGCA715300.034.4178055
TATCAAC721450.034.246052
AACGCAG740950.033.342216
ACGCAGA831150.029.308897
CGCAGAG830350.029.0026258
GTGGTAT102200.027.537481
GCAGAGT895250.026.0458329
GTACATG463150.024.3158281
TACATGG458900.022.9081922
TGGTATC119300.022.6972622
AGAGTAC829050.021.71788810-11
ACATGGG476150.021.3703613
GAGTACT621650.021.04699712-13
CAGAGTA874400.020.54206710-11
AGTACTT648200.020.5113912-13
GTACTTT671250.019.2201714-15
Produced by FastQC (version 0.11.5)