FastQCFastQC Report
Thu 2 Jun 2016
DRR015388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494864
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295340.39405651656921326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246030.3282647957321173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136490.1821113765373194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150550.058.732471
GTATCAA289300.041.3393061
ATCAACG354350.033.32553
TCAACGC356700.033.1818244
CAACGCA368800.032.0191275
TATCAAC372550.031.7718142
AACGCAG382550.031.1381136
TATAACG6600.030.3385622
ACGCAGA421750.027.92037
CGCAGAG422300.027.5369538
GTGGTAT55600.027.1737481
GCAGAGT442050.025.4102219
GTACATG236600.024.7542651
TAACGCA7950.024.6129914
TACATGG234400.023.7634812
TGGTATC61000.023.2761422
ACATGGG241050.022.0310633
CAGAGTA436450.021.75973710-11
GAGTACT309950.021.73805612-13
AGAGTAC411850.020.88330710-11
Produced by FastQC (version 0.11.5)