FastQCFastQC Report
Thu 2 Jun 2016
DRR015388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494864
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298030.3976456410683369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226260.30188673203409694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154090.20559412418957837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136500.058.9458961
GTATCAA271250.043.4286581
ATCAACG346100.033.726983
TCAACGC347050.033.5948684
CAACGCA357500.032.7074935
TATCAAC361250.032.3255732
AACGCAG370500.031.7065876
ACGCAGA423750.027.423427
CGCAGAG425350.027.1309788
GTGGTAT52300.026.206831
TAACGCA8150.025.1270054
GCAGAGT450900.024.8362129
TGGTATC52600.024.7444442
TATAACG7650.024.3904512
GTACATG219300.024.0862431
TACATGG216050.022.9388182
TACCTGG53600.022.8394132
AGAGTAC416450.021.35769310-11
ACATGGG223300.021.2358973
ATAACGC9950.021.0390623
Produced by FastQC (version 0.11.5)