FastQCFastQC Report
Thu 2 Jun 2016
DRR015387_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015387_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9724599
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433540.4458178686853823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345820.35561363507122506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194390.19989513192266334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182700.057.657931
GTATCAA360450.043.540381
ATCAACG437300.035.4493183
TCAACGC438350.035.434154
CAACGCA453300.034.3151785
TATCAAC459250.033.8441772
AACGCAG471950.033.0651866
ACGCAGA529050.029.2556957
GTGGTAT65350.029.2005081
CGCAGAG532400.028.8834638
TATAACG9000.028.8169252
GCAGAGT566250.026.5121579
TGGTATC71700.025.2568512
GTACATG289050.023.6184081
TACATGG286550.022.3889642
GAGTACT399650.021.99221212-13
TAACGCA12100.021.808324
CAGAGTA552800.021.77120410-11
AGAGTAC524400.021.42776910-11
ACATGGG294500.021.2438243
Produced by FastQC (version 0.11.5)