FastQCFastQC Report
Thu 2 Jun 2016
DRR015387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9724599
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471560.48491459647847684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364270.3745861397472533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232430.2390124261164908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188100.057.738441
GTATCAA374650.043.482991
ATCAACG469050.034.3530543
TCAACGC471650.034.16354
CAACGCA483350.033.397125
TATCAAC489200.033.11492
AACGCAG503400.032.2109226
GTGGTAT69500.030.0867651
TATAACG10000.030.0502322
ACGCAGA571950.028.0476957
CGCAGAG574800.027.80578
TGGTATC72850.027.3121452
TAACGCA12700.026.1710324
GCAGAGT611050.025.4836359
GTACATG274450.023.1889341
TACATGG271450.022.0566652
TACCTGG63500.022.012462
AGAGTAC568300.021.62771610-11
ATAACGC15400.020.9914073
CAGAGTA596550.020.91627310-11
Produced by FastQC (version 0.11.5)