FastQCFastQC Report
Thu 2 Jun 2016
DRR015386_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015386_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570016
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321940.3756585751998596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265100.3093343116278896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146980.1715049306792426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150900.060.3021971
GTATCAA297200.042.4020841
TCAACGC358150.034.641634
ATCAACG359850.034.466743
CAACGCA369250.033.537885
TATCAAC375800.033.0886232
AACGCAG384050.032.3046766
ACGCAGA428950.028.5838077
CGCAGAG428700.028.3670048
GTGGTAT56550.028.02681
GCAGAGT459900.025.4919959
TGGTATC57300.025.1710032
GTACATG229250.023.6607931
TACATGG225850.022.4218222
CAGAGTA445550.021.8867410-11
GAGTACT324500.021.77262512-13
ACATGGG232400.020.9872023
AGAGTAC420500.020.96726610-11
GTACTTT349300.019.94077714-15
AGTACTT336450.019.56612-13
Produced by FastQC (version 0.11.5)