FastQCFastQC Report
Thu 2 Jun 2016
DRR015386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570016
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342130.39921745770369627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242050.2824382124840841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199280.2325316545500032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130700.054.926481
GTATCAA272450.044.77391
ATCAACG360450.033.6120873
TCAACGC361300.033.482614
CAACGCA370200.032.602835
TATCAAC375850.032.3923572
AACGCAG385800.031.4489356
ACGCAGA446650.026.8071987
CGCAGAG448400.026.37788
GCAGAGT474950.024.1539069
GTGGTAT54350.023.1318661
TGGTATC55250.022.2498532
AGAGTAC440900.022.17985510-11
GTACATG197900.021.3370861
AGTACTT369250.020.80818212-13
TACATGG195650.020.5017872
TATAACG7000.020.1631952
GAGTACT354200.019.5340112-13
CAGAGTA466650.018.99103510-11
ACATGGG205000.018.3895953
Produced by FastQC (version 0.11.5)