FastQCFastQC Report
Thu 2 Jun 2016
DRR015385_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015385_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12416179
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT743010.598420818514295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560750.4516284760392066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324370.26124784444554155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124220.10004688237822604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279850.060.7562141
GTATCAA570200.046.0821
ATCAACG721750.036.004033
TCAACGC725400.035.8266874
TATCAAC748300.034.842122
CAACGCA766550.033.8790135
AACGCAG788250.033.0271536
GTGGTAT85200.031.9148461
ACGCAGA863350.029.621977
CGCAGAG867650.029.228718
GTACATG570900.027.1174661
GCAGAGT915200.026.972299
TGGTATC98800.026.1127052
TACATGG566800.026.098342
ACATGGG600700.023.8528773
CAGAGTA890250.022.1249110-11
GAGTACT608650.021.67999812-13
AGAGTAC859200.021.2247910-11
CATGGGG328500.021.177184
CATGGGA257200.020.8563774
Produced by FastQC (version 0.11.5)