FastQCFastQC Report
Thu 2 Jun 2016
DRR015385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12416179
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817780.658640633322055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT571350.46016572409273415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411370.33131771054524906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266850.059.1004681
GTATCAA564250.047.8352281
ATCAACG753800.035.5951423
TCAACGC758900.035.3379364
TATCAAC779000.034.5371862
CAACGCA785400.034.0904585
AACGCAG811250.033.04886
GTGGTAT85350.029.8359151
ACGCAGA914800.028.909527
CGCAGAG916100.028.6196388
GTACATG541800.026.6196881
GCAGAGT968800.026.3887169
TACATGG537300.025.7358422
TGGTATC99450.025.2265152
ACATGGG575350.023.1870733
AGAGTAC914000.022.10079210-11
AGTACTT674200.021.49351312-13
GAGTACT668800.021.1907812-13
CAGAGTA944900.020.99277910-11
CATGGGG317000.020.4675584
Produced by FastQC (version 0.11.5)