FastQCFastQC Report
Thu 2 Jun 2016
DRR015384_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015384_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10229931
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514120.5025644845502868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408460.3992793304275464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233170.22792920108649803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210100.061.664871
GTATCAA424650.043.7579351
ATCAACG520450.035.2915083
TCAACGC522900.035.1503684
TATCAAC534850.034.392382
CAACGCA546900.033.5823865
AACGCAG561200.032.823956
GTGGTAT67650.029.6964861
ACGCAGA619900.029.282767
CGCAGAG622900.028.8785118
GCAGAGT656550.026.545469
GTACATG389950.025.68921
TGGTATC74650.025.1715952
TACATGG393200.024.3225822
ACATGGG402700.022.7658523
GAGTACT429200.022.4981412-13
CAGAGTA638100.022.3087610-11
AGAGTAC606000.021.30207410-11
GTACTTT464750.020.61608114-15
TAACGCA9500.020.5926364
Produced by FastQC (version 0.11.5)