FastQCFastQC Report
Thu 2 Jun 2016
DRR015384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10229931
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541530.5293584091622905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397120.38819421167161344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277090.27086204198249236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200300.061.6188961
GTATCAA411250.045.3441931
ATCAACG535900.034.7215043
TCAACGC540100.034.400914
TATCAAC547800.034.0669942
CAACGCA556100.033.426885
AACGCAG572600.032.502126
ACGCAGA650700.028.1807047
CGCAGAG657350.027.556228
GTGGTAT68450.027.4892221
TGGTATC70450.025.8560942
GTACATG362900.025.3349511
GCAGAGT693350.025.2688949
TACATGG369500.024.082082
ACATGGG379300.022.4993863
AGAGTAC639950.021.73138810-11
GAGTACT475150.021.7114712-13
TAACGCA8700.021.460934
CAGAGTA673600.021.0578910-11
AGTACTT491150.020.62894412-13
Produced by FastQC (version 0.11.5)