FastQCFastQC Report
Thu 2 Jun 2016
DRR015383_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015383_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15946011
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366070.22956838547270536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292190.18323704906512356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174340.1093314183716542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236050.051.729581
GTATCAA436050.039.84781
TCAACGC515450.033.1265534
ATCAACG516350.033.081153
CAACGCA547600.031.1726765
TATCAAC560400.030.6675722
AACGCAG570300.030.0675096
GTACATG499850.029.9141961
TACATGG489400.028.7664892
ACGCAGA621250.027.228127
CGCAGAG628450.026.6700348
ACATGGG515600.026.34283
GCAGAGT686400.023.7736639
CATGGGG321200.021.714554
AGAGTAC619150.020.04076410-11
CAGAGTA673550.020.00601810-11
CATGGGA192650.019.9559294
GAGTACT434450.019.82760412-13
GTGGTAT98750.018.8639351
GTACTTT455800.018.6397714-15
Produced by FastQC (version 0.11.5)