FastQCFastQC Report
Thu 2 Jun 2016
DRR015383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15946011
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383730.24064325554522698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292890.1836760303250763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206090.12924235409093848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA235550.051.177361
GTATCAA451450.041.1435931
ATCAACG551150.033.187993
TCAACGC554200.032.914754
CAACGCA573150.031.8592625
TATCAAC599900.030.7339082
AACGCAG594750.030.7326226
GTACATG477000.030.2769431
TACATGG479400.028.5712132
ACGCAGA665250.027.0168327
CGCAGAG673100.026.43128
ACATGGG502150.026.2067553
GCAGAGT736050.023.6048289
CATGGGG320600.021.3188274
AGAGTAC664550.020.85479410-11
GAGTACT480250.019.80332812-13
CAGAGTA717250.019.51599510-11
AGTACTT492100.019.31720712-13
GTGGTAT104050.019.0028511
CATGGGA188250.018.7219944
Produced by FastQC (version 0.11.5)