FastQCFastQC Report
Thu 2 Jun 2016
DRR015382_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015382_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10707434
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540060.5043785467181026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426080.397929139698643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230150.21494412199972468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA240500.056.495871
GTATCAA471400.043.3652421
TCAACGC578400.034.989534
ATCAACG578250.034.953683
TATCAAC593450.034.135092
CAACGCA597050.033.925945
AACGCAG617200.032.936296
ACGCAGA687200.029.1443467
CGCAGAG686450.028.8713028
GCAGAGT723600.026.5572019
GTGGTAT91600.024.8629721
GTACATG392900.023.0816971
GAGTACT465950.022.54932812-13
TGGTATC99350.022.350232
TACATGG391550.022.1263482
CAGAGTA705350.021.92978110-11
AGAGTAC655400.021.48395510-11
GTACTTT500350.020.75874914-15
TATAACG11950.020.5615522
ACATGGG410700.020.3523463
Produced by FastQC (version 0.11.5)