FastQCFastQC Report
Thu 2 Jun 2016
DRR015382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10707434
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT574760.5367859376952498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417050.38949574659997904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291340.2720913339274377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239400.056.13521
GTATCAA475400.043.3224261
ATCAACG611450.033.4376643
TCAACGC614350.033.2352034
TATCAAC624150.032.7645842
CAACGCA629750.032.4789775
AACGCAG649800.031.5321166
ACGCAGA738050.027.4407187
CGCAGAG737000.027.1400478
GCAGAGT776850.025.1009569
GTGGTAT94950.023.3173241
GTACATG360100.022.480281
AGAGTAC709650.021.91217210-11
GAGTACT525850.021.54402412-13
TGGTATC100650.021.5349452
TACATGG360850.021.2503452
AGTACTT539700.021.03752712-13
CAGAGTA758150.020.60645910-11
GTACTTT559350.019.87967714-15
ACATGGG383450.019.3684923
Produced by FastQC (version 0.11.5)