FastQCFastQC Report
Thu 2 Jun 2016
DRR015381_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015381_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8489340
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408330.480991455166126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352940.4157449224556915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181440.21372686215889572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198050.063.0888631
GTATCAA379850.041.144361
GTGGTAT64450.034.8438151
ATCAACG462350.033.411783
TCAACGC464800.033.2229424
CAACGCA481250.032.0865
TATCAAC484500.031.9875972
TGGTATC66650.031.882142
AACGCAG496800.031.2556956
ACGCAGA542800.028.1291477
CGCAGAG540950.027.905758
GCAGAGT565300.025.8737019
GAGTACT395350.023.00878312-13
CAGAGTA554400.022.89483510-11
GTACATG269200.021.6927531
GTACTTT419950.021.34151314-15
AGAGTAC527400.020.97445510-11
TACATGG269050.020.4974752
AGTACTT401050.020.14644612-13
ACATGGG274900.019.316433
Produced by FastQC (version 0.11.5)