FastQCFastQC Report
Thu 2 Jun 2016
DRR015381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8489340
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418110.4925117853684739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334840.3944240659462337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216220.25469588919751124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175850.060.810781
GTATCAA357100.044.682361
ATCAACG464600.034.0794033
TCAACGC467800.033.8560184
CAACGCA477350.033.1813285
GTGGTAT57850.033.1580161
TATCAAC483300.032.8173262
AACGCAG495700.032.0071566
TGGTATC61900.029.7205772
ACGCAGA552750.028.0110177
CGCAGAG551850.027.8010398
GCAGAGT583150.025.6516089
AGAGTAC540650.022.41966210-11
GAGTACT418350.021.96203212-13
GTACATG257650.021.8750671
CAGAGTA570750.021.32102610-11
AGTACTT430350.021.13286212-13
TACATGG253950.020.6571672
TATAACG9550.020.503672
GTACTTT445950.020.3835214-15
Produced by FastQC (version 0.11.5)