FastQCFastQC Report
Thu 2 Jun 2016
DRR015380_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015380_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081757
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323810.40066782507813586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251650.3113803099994222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133470.16514973167344674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151450.060.3883861
GTATCAA309200.045.1344641
TCAACGC373250.036.9468424
ATCAACG373950.036.8673363
CAACGCA386700.035.626265
TATCAAC396250.034.9303252
AACGCAG400200.034.5267876
ACGCAGA441500.030.864177
CGCAGAG440950.030.582818
GCAGAGT464200.027.9517299
GTGGTAT53450.027.3537161
TGGTATC58650.023.894362
GTACATG257900.022.9942191
GAGTACT315250.022.76543212-13
CAGAGTA459600.022.33666410-11
TACATGG254650.021.7986132
GTACTTT329850.021.41362614-15
AGAGTAC434300.021.40660510-11
ACATGGG257600.020.6658233
AGTACTT323750.019.96149812-13
Produced by FastQC (version 0.11.5)