FastQCFastQC Report
Thu 2 Jun 2016
DRR015380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081757
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330590.40905708993725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200520.24811436424035022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188410.23312999883564922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112200.054.9663241
GTATCAA259950.048.8510441
ATCAACG362850.034.4967843
TCAACGC364900.034.2156334
CAACGCA377100.033.180265
TATCAAC383300.032.8820042
AACGCAG391450.032.0677726
ACGCAGA454100.027.1918267
CGCAGAG454050.026.8638678
GCAGAGT480200.024.6405819
AGAGTAC446650.023.02711710-11
AGTACTT353850.022.00512712-13
GTACATG206900.021.1339971
TATAACG8500.020.891842
CAGAGTA472450.020.267019
TACATGG207950.020.2322442
TAACGCA8200.019.9903164
GAGTACT339950.019.63179812-13
ATAACGC12550.019.5921043
GTGGTAT50450.018.7790281
Produced by FastQC (version 0.11.5)