FastQCFastQC Report
Thu 2 Jun 2016
DRR015379_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015379_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12357605
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194830.15765999965203614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163920.13264706227460743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212150.043.750541
GTATCAA357500.035.4662671
GTACATG433350.030.5407071
TCAACGC413950.030.1606034
ATCAACG414700.030.1072753
TACATGG424200.029.1755892
CAACGCA437150.028.5800725
TATCAAC448800.028.0123442
AACGCAG455850.027.5274246
ACATGGG443500.027.05443
ACGCAGA493400.024.8515197
CGCAGAG502600.024.1793548
CATGGGG262900.022.4640434
CATGGGA172350.021.9378244
GCAGAGT542750.021.4748639
GAGTACT280300.018.77032912-13
CAGAGTA525550.018.52468510-11
AGAGTAC457650.018.23624210-11
GTACTTT282650.018.1718714-15
GAGTACA311550.018.0388851
Produced by FastQC (version 0.11.5)