FastQCFastQC Report
Thu 2 Jun 2016
DRR015379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12357605
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200360.16213497680173464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165270.1337395069675718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205600.041.168241
GTATCAA348800.037.000691
ATCAACG417700.030.4542773
TCAACGC419800.030.2692724
GTACATG410000.029.933221
CAACGCA434550.029.208235
TACATGG401650.028.8487072
AACGCAG452500.028.0790716
TATCAAC454750.028.0731852
ACATGGG419300.026.5376643
ACGCAGA504650.024.7445587
CGCAGAG513600.024.056218
GCAGAGT554800.021.4963879
CATGGGG251900.021.2732054
CATGGGA163900.020.8337254
AGAGTAC467150.019.67097710-11
GAGTACT298050.018.59449212-13
GTACTTT300600.018.06626514-15
AGTACTT301900.018.0559312-13
CAGAGTA535850.017.78164310-11
Produced by FastQC (version 0.11.5)