FastQCFastQC Report
Thu 2 Jun 2016
DRR015378_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015378_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616063
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405820.32166928779604226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317470.25163951701889886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198110.15702997044323574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190250.058.615121
GTATCAA384450.043.023851
ATCAACG463650.035.0447463
TCAACGC463250.035.01334
CAACGCA486950.033.2897155
TATCAAC497750.032.8175772
AACGCAG506300.032.161216
ACGCAGA555050.028.8446857
CGCAGAG554250.028.7713978
GTACATG358300.028.5806671
TACATGG350600.026.8646892
GCAGAGT598450.025.7706079
ACATGGG356750.025.2919373
GTGGTAT78000.023.4192331
CATGGGG211950.021.5520844
CAGAGTA593050.021.24917810-11
AGAGTAC562000.021.15630510-11
TGGTATC79550.021.0489792
GAGTACT446950.020.63738812-13
GTACTTT474050.019.2563814-15
Produced by FastQC (version 0.11.5)