FastQCFastQC Report
Thu 2 Jun 2016
DRR015378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616063
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420320.3331625721907064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309200.2450843817124249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236970.1878319726209357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186300.059.373791
GTATCAA390050.044.1616551
ATCAACG493400.034.748573
TCAACGC496500.034.4946484
CAACGCA511150.033.561165
TATCAAC526350.032.6684462
AACGCAG532150.032.2618986
ACGCAGA601850.028.2301657
CGCAGAG601650.028.0048188
GTACATG348200.027.0309011
TACATGG343000.025.5435942
GCAGAGT641900.025.507749
ACATGGG348600.023.8269543
AGAGTAC614150.021.6293310-11
GTGGTAT75350.021.5845951
CAGAGTA641000.020.49259610-11
TGGTATC75050.020.3872992
AGTACTT504950.019.98427212-13
GAGTACT488200.019.9615112-13
CATGGGG198850.019.78594
Produced by FastQC (version 0.11.5)