FastQCFastQC Report
Thu 2 Jun 2016
DRR015377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10708660
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282700.26399194670481646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229970.2147514254818063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127400.11896913339297353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162700.054.4745981
GTATCAA310000.041.6594281
ATCAACG369750.034.2324033
TCAACGC369950.034.1889844
CAACGCA386050.032.7985085
TATCAAC392900.032.4239922
AACGCAG401650.031.5925876
ACGCAGA450600.027.7565827
CGCAGAG450750.027.5858468
GTACATG316600.026.5421711
TACATGG320250.025.1463322
ACATGGG319900.024.604833
GCAGAGT493950.024.5008459
GTGGTAT74650.021.5258641
GAGTACT330250.020.71969612-13
CAGAGTA482150.020.71713310-11
CATGGGG196800.020.2055554
AGAGTAC453700.020.19115810-11
TGGTATC73550.019.6713852
GTACTTT361150.018.66976714-15
Produced by FastQC (version 0.11.5)