FastQCFastQC Report
Thu 2 Jun 2016
DRR015377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10708660
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271690.2537105482852196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210040.1961403200773953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143770.13425582659268293No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149950.053.9655081
GTATCAA289550.041.9996571
ATCAACG362050.033.0506253
TCAACGC363050.032.921994
CAACGCA376050.031.7959795
TATCAAC384700.031.1756612
AACGCAG392550.030.5638166
GTACATG296450.026.4966431
ACGCAGA451900.026.2879647
CGCAGAG455050.025.9260188
TACATGG298200.024.9918672
ACATGGG307650.023.42563
GCAGAGT497200.023.2984929
GTGGTAT69750.020.665791
AGAGTAC453800.020.54366910-11
TGGTATC68850.019.8909262
GAGTACT336450.019.73037112-13
CAGAGTA486000.019.34169810-11
AGTACTT351350.018.84832612-13
CATGGGG188750.018.7054084
Produced by FastQC (version 0.11.5)