FastQCFastQC Report
Thu 2 Jun 2016
DRR015376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10685807
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391890.3667387966112433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310650.2907127182813614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175800.16451728914811956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213150.054.9437181
GTATCAA398200.042.1325451
ATCAACG488500.033.9201743
TCAACGC492350.033.6549344
TATCAAC511650.032.5457952
CAACGCA516550.032.034185
AACGCAG533150.031.1818476
GTACATG451450.027.9074421
ACGCAGA594800.027.6056947
TACATGG445950.027.3933332
CGCAGAG596700.027.3881688
ACATGGG459200.025.8100323
CATGGGT161950.025.5648444
GCAGAGT635450.025.0092079
GTGGTAT85400.022.5980821
GAGTACT393850.021.6942912-13
CAGAGTA620500.021.26360310-11
TGGTATC85150.021.1591052
CATGGGG227750.020.316334
AGAGTAC584750.020.24110810-11
Produced by FastQC (version 0.11.5)