FastQCFastQC Report
Thu 2 Jun 2016
DRR015376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10685807
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383380.35877496196590486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288740.27020888548707644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197920.1852176443014552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200200.054.0052151
GTATCAA390700.042.863971
ATCAACG495600.033.5360573
TCAACGC501950.033.039294
TATCAAC520200.031.9763932
CAACGCA521000.031.8137665
AACGCAG536050.030.9375446
GTACATG433800.027.6262511
TACATGG433350.026.9198632
ACGCAGA609950.026.8237257
CGCAGAG612050.026.6201848
ACATGGG448450.024.9684283
GCAGAGT654700.024.3304149
CATGGGT157650.024.2714464
GTGGTAT90000.021.441211
AGAGTAC597900.020.80509410-11
GAGTACT407400.020.6274512-13
CAGAGTA636100.020.14935710-11
TGGTATC90100.020.0979582
AGTACTT417300.020.01815412-13
Produced by FastQC (version 0.11.5)