FastQCFastQC Report
Thu 2 Jun 2016
DRR015375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531602
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171670.18010613535898792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146810.15402447563379168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126600.052.209281
GTATCAA227250.039.762291
TCAACGC274000.032.512064
ATCAACG274600.032.4741553
CAACGCA290300.030.6395265
GTACATG326000.030.203251
TATCAAC298500.030.0266232
AACGCAG297750.029.9951346
TACATGG324950.029.0806792
ACATGGG330450.027.4537543
ACGCAGA345250.025.604677
CGCAGAG348500.025.1831178
CATGGGT107650.024.4707264
GCAGAGT386700.022.1071459
CATGGGG193550.021.3204544
GAGTACA226750.020.2361551
GTGGTAT60400.019.7459981
TATACCG8000.019.3362055
CAGAGTA377200.019.09969510-11
AGAGTAC353950.018.84395810-11
Produced by FastQC (version 0.11.5)