FastQCFastQC Report
Thu 2 Jun 2016
DRR015375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531602
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162820.17082123235947116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129310.1356644979511314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118450.051.645261
GTATCAA219550.041.763741
TCAACGC279250.032.406924
ATCAACG279850.032.3862153
CAACGCA290200.031.1841225
AACGCAG298450.030.3983336
TATCAAC302600.030.1167562
GTACATG304650.030.0676361
TACATGG304600.029.1870922
ACATGGG313250.027.5532233
ACGCAGA348400.025.7267117
CGCAGAG355700.025.0708128
CATGGGT102850.024.1979854
GCAGAGT389850.022.349489
CATGGGG180700.021.0496224
AGAGTAC359200.020.01321810-11
CAGAGTA376200.018.63717310-11
GAGTACA212050.018.027791
AGTACTT255050.018.02632512-13
GTGGTAT58150.017.9648061
Produced by FastQC (version 0.11.5)