FastQCFastQC Report
Thu 2 Jun 2016
DRR015374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9871498
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282510.2861875674796267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223950.22686526401565396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126120.12776176422261343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146450.054.5544971
GTATCAA266350.040.2001231
ATCAACG319450.032.7144053
TCAACGC321300.032.6391564
CAACGCA338850.030.9352575
TATCAAC343000.030.68052
AACGCAG348150.030.2526446
GTACATG282300.026.7533591
ACGCAGA396650.026.11747
TACATGG283300.025.6471612
CGCAGAG403000.025.5816888
ACATGGG287950.024.2533783
GTGGTAT70050.023.1358071
GCAGAGT435800.023.0612499
TGGTATC70000.020.6685522
CAGAGTA421900.020.12204410-11
GAGTACT296700.019.92639212-13
CATGGGG180150.019.9008064
AGAGTAC400800.019.46732910-11
GAGTACA206400.018.1523531
Produced by FastQC (version 0.11.5)