FastQCFastQC Report
Thu 2 Jun 2016
DRR015374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9871498
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269510.27301834027621746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201890.20451809846894564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140740.1425720797390629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141500.053.0494541
GTATCAA268800.041.4390641
ATCAACG329800.033.3025973
TCAACGC330500.033.2182964
CAACGCA343250.031.9578955
TATCAAC355800.031.0224692
AACGCAG353150.031.0104716
GTACATG266850.027.309211
ACGCAGA412150.026.3504857
TACATGG266550.025.9451642
CGCAGAG417900.025.8463888
ACATGGG268700.024.6030773
GCAGAGT451650.023.4519569
CATGGGG173750.020.4773354
AGAGTAC411950.020.46581310-11
GAGTACT308800.019.75093512-13
CAGAGTA441350.019.46332210-11
TATAACG8200.019.4198422
GTGGTAT69650.019.1308021
AGTACTT321450.019.00906212-13
Produced by FastQC (version 0.11.5)