FastQCFastQC Report
Thu 2 Jun 2016
DRR015373_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015373_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477358
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278110.26543905438756604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222760.21261085094162097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126820.12104196496864955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154850.052.0345271
GTATCAA282950.041.13871
TCAACGC335950.034.1005824
ATCAACG336500.034.0718883
CAACGCA352500.032.4221085
TATCAAC360300.031.872352
AACGCAG364450.031.5462686
GTACATG302500.027.1782741
ACGCAGA420900.027.0774337
CGCAGAG423600.026.7544758
TACATGG297550.026.361272
ACATGGG298800.025.0932753
GCAGAGT462650.024.034079
TATAACG6950.022.9124892
GTGGTAT68550.021.981131
CATGGGG190700.020.636984
CAGAGTA445150.020.45122710-11
GAGTACT313550.019.90792512-13
AGAGTAC421750.019.83294510-11
TGGTATC74800.018.43022
Produced by FastQC (version 0.11.5)