FastQCFastQC Report
Thu 2 Jun 2016
DRR015373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477358
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256420.24473727059817946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195410.18650694192180892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132470.1264345458082085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138750.052.9616661
GTATCAA267100.041.9253461
TCAACGC332250.033.2491724
ATCAACG333350.033.125813
CAACGCA345800.032.012115
TATCAAC352200.031.3399732
AACGCAG358550.030.924526
GTACATG277600.026.3069761
ACGCAGA421300.026.0377187
CGCAGAG424550.025.6883588
TACATGG280550.024.780512
ACATGGG285200.023.3554783
GCAGAGT469550.022.9358929
AGAGTAC421250.020.19225310-11
GTGGTAT63050.019.6057421
CAGAGTA445850.019.27713210-11
AGTACTT320250.018.49073812-13
GAGTACT315050.018.4348912-13
CATGGGG181150.018.1089844
TATAACG7450.017.7108292
Produced by FastQC (version 0.11.5)