FastQCFastQC Report
Thu 2 Jun 2016
DRR015372_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015372_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11111871
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319520.2875483345693988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251660.22647851113462353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140460.12640535513776213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231650.042.8364071
GTATCAA389500.035.713691
ATCAACG453650.030.2579823
TCAACGC454400.030.197894
CAACGCA475800.028.8109995
TATCAAC477650.028.7762
AACGCAG487100.028.245376
GTACATG334050.026.094331
ACGCAGA538800.025.2646757
CGCAGAG542200.024.971998
TACATGG335150.024.7342412
ACATGGG334850.023.8458253
GCAGAGT579550.022.7267519
GAGTACT343950.021.58751112-13
AGAGTAC486400.019.96550610-11
CAGAGTA569300.019.29983910-11
GTACTTT384150.019.12119314-15
CATGGGG204100.018.9701234
CATGGGT90800.018.9404184
AGTACTT364750.018.32332212-13
Produced by FastQC (version 0.11.5)