FastQCFastQC Report
Thu 2 Jun 2016
DRR015372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11111871
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295550.2659768098459746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220930.19882340246750527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148720.13383884676127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209750.040.2411231
GTATCAA367400.038.3600461
ATCAACG448250.031.0290223
TCAACGC447750.030.9925424
CAACGCA463700.029.9166725
TATCAAC471950.029.6154382
AACGCAG474850.029.2141976
ACGCAGA537900.025.7137247
CGCAGAG539850.025.4017168
GTACATG315250.025.2256661
TACATGG322150.023.5293462
ACATGGG316900.023.1438583
GCAGAGT583250.022.9968139
AGAGTAC487950.021.46936610-11
GAGTACT347650.020.579912-13
AGTACTT367750.019.83860812-13
GTACTTT383750.018.4482914-15
CATGGGG192050.018.3603234
CAGAGTA569800.018.34543410-11
CATGGGT93000.018.1992284
Produced by FastQC (version 0.11.5)