FastQCFastQC Report
Thu 2 Jun 2016
DRR015371_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015371_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10533976
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254320.24142830779185373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204550.19418119046407548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114240.1084490794359129No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158650.053.2262651
GTATCAA288100.040.7186471
ATCAACG345150.033.4422573
TCAACGC346900.033.3391274
CAACGCA362300.031.8968895
TATCAAC365500.031.854442
AACGCAG373050.031.1362826
ACGCAGA419900.027.1637297
CGCAGAG423650.026.7514558
GTACATG300100.026.0754641
TACATGG303450.024.7241042
GTGGTAT65150.024.5952031
ACATGGG302550.023.8650723
GCAGAGT469350.023.4487679
GAGTACT307550.020.59218212-13
CAGAGTA453100.020.39363910-11
TGGTATC72350.020.0603832
AGAGTAC427350.019.56241410-11
CATGGGG179850.018.7705254
GTACTTT343500.018.19863514-15
Produced by FastQC (version 0.11.5)