FastQCFastQC Report
Thu 2 Jun 2016
DRR015371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10533976
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234760.22285982045146105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178660.16960357608561097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121440.11528410545078135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143900.051.5825161
GTATCAA274050.042.8337141
ATCAACG335900.034.770333
TCAACGC339750.034.3495334
CAACGCA352450.033.1505285
TATCAAC360400.032.520262
AACGCAG364650.032.1038066
ACGCAGA421350.027.4273387
CGCAGAG423800.027.0540648
GTACATG281600.025.3546371
GCAGAGT464400.024.0915899
TACATGG283850.023.9471932
ACATGGG286850.023.030573
GTGGTAT64500.020.9366741
AGAGTAC427500.020.55690610-11
GAGTACT312600.019.6709912-13
CAGAGTA451750.019.66491110-11
AGTACTT324450.019.13484212-13
TGGTATC70400.017.8373092
GTACTTT342650.017.7334114-15
Produced by FastQC (version 0.11.5)