FastQCFastQC Report
Thu 2 Jun 2016
DRR015370_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015370_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12098611
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301480.24918562965616464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246000.20332912596330274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137400.11356675572096665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182300.053.1321681
GTATCAA341200.040.4609381
ATCAACG401250.033.8694273
TCAACGC403250.033.690024
CAACGCA420500.032.297155
TATCAAC433400.031.5459232
AACGCAG438300.031.058186
ACGCAGA492100.027.3019817
CGCAGAG493050.027.0186848
GTACATG335050.026.2328361
TACATGG331250.024.9292052
GCAGAGT538400.024.0583869
GTGGTAT76500.023.5616841
ACATGGG338550.023.3703083
GAGTACT360700.020.4654312-13
CAGAGTA521250.020.4463510-11
AGAGTAC484350.020.0079510-11
TGGTATC84650.019.4567052
GTACTTT390600.018.68333414-15
CATGGGG211750.018.6501454
Produced by FastQC (version 0.11.5)