FastQCFastQC Report
Thu 2 Jun 2016
DRR015370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12098611
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287990.2380355893746811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220740.18245069619975382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147240.12169992075949874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161450.051.6794621
GTATCAA312650.042.4305151
ATCAACG385000.034.0705873
TCAACGC387950.033.8818784
CAACGCA402800.032.7118265
TATCAAC416800.031.6239782
AACGCAG420900.031.3159266
ACGCAGA486100.026.8441247
CGCAGAG485800.026.579738
GTACATG310600.026.3930111
TACATGG310950.024.5379452
GCAGAGT527350.023.7263669
ACATGGG315000.023.3847083
GTGGTAT72700.021.8994561
AGAGTAC481750.020.49913610-11
GAGTACT360400.019.47953812-13
CAGAGTA511350.019.43709210-11
TGGTATC80800.019.0327262
AGTACTT373650.018.84965912-13
CATGGGG199100.018.2816094
Produced by FastQC (version 0.11.5)