FastQCFastQC Report
Thu 2 Jun 2016
DRR015369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096227
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257690.2552339601714581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207870.20588879390291046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109010.10797102719659532No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163150.051.392541
GTATCAA296800.040.412361
ATCAACG355900.033.2254943
TCAACGC358950.032.8794754
TATCAAC381050.031.259262
CAACGCA381050.030.9725445
AACGCAG392550.030.1810866
ACGCAGA434650.026.7761177
GTACATG336000.026.7698061
CGCAGAG438750.026.2770258
TACATGG334350.025.6372012
ACATGGG343850.024.2409483
GCAGAGT473650.023.7068759
GTGGTAT73650.022.4352761
GAGTACT285850.020.28542312-13
TGGTATC75400.020.274912
CAGAGTA458250.020.26389510-11
AGAGTAC433050.019.47843710-11
CATGGGT98600.019.1957594
CATGGGG201150.019.1580924
Produced by FastQC (version 0.11.5)