FastQCFastQC Report
Thu 2 Jun 2016
DRR015369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096227
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270620.26804072452015987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204530.2025806273967493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127390.12617584767061993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156850.051.416891
GTATCAA296650.041.9169351
ATCAACG360100.034.03843
TCAACGC361950.033.864424
CAACGCA378450.032.3999945
TATCAAC385950.031.9825732
AACGCAG392800.031.2742526
ACGCAGA448000.027.1059887
CGCAGAG451800.026.6363128
GTACATG314200.026.0014441
TACATGG320400.024.3395752
GTGGTAT69450.024.1047061
GCAGAGT491500.023.9759169
ACATGGG325450.023.025233
TGGTATC71200.021.9183982
GAGTACT303450.019.80681612-13
AGAGTAC444500.019.7809310-11
CAGAGTA474500.019.76724210-11
AGTACTT305050.018.8005612-13
GTACTTT318600.018.52222614-15
Produced by FastQC (version 0.11.5)