FastQCFastQC Report
Thu 2 Jun 2016
DRR015368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9677499
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265450.27429607587662885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217230.22446915261887393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113100.11686903816781587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153150.055.4874571
GTATCAA288650.040.6674841
ATCAACG337050.034.17923
TCAACGC338650.034.031154
CAACGCA353500.032.614425
TATCAAC357600.032.3673822
AACGCAG367150.031.5257956
ACGCAGA415500.027.4959167
CGCAGAG419450.027.0200428
GTACATG294050.025.5405541
GCAGAGT457200.024.2616319
TACATGG296400.024.099582
ACATGGG300350.022.813393
GTGGTAT67950.022.5739731
GAGTACT296050.021.24631312-13
CAGAGTA443400.021.01956710-11
TGGTATC68500.020.3908712
AGAGTAC420450.019.52116210-11
CATGGGG184650.019.441074
GTACTTT322000.019.26561214-15
Produced by FastQC (version 0.11.5)