FastQCFastQC Report
Thu 2 Jun 2016
DRR015368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9677499
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270940.27996902918822314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212740.21982952413634968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127100.13133558577479576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147450.054.829781
GTATCAA280300.042.7030371
ATCAACG343000.034.4750983
TCAACGC343650.034.3966564
CAACGCA356600.033.070985
TATCAAC362100.032.7571332
AACGCAG369700.031.9729776
ACGCAGA427200.027.4351927
CGCAGAG431400.026.9571558
GTACATG277100.025.341761
GCAGAGT469250.024.3083029
TACATGG282550.023.6387272
ACATGGG288550.022.35883
GTGGTAT65300.021.437681
GAGTACT317800.020.5448312-13
CAGAGTA454950.020.4019210-11
AGAGTAC433550.019.84526810-11
GTACTTT340550.018.91183314-15
TATAACG7250.018.8268052
AGTACTT324450.018.53908312-13
Produced by FastQC (version 0.11.5)